Journal: Advanced Healthcare Materials

Article Title: Design of Recombinant Spider Silk Proteins for Cell Type Specific Binding

doi: 10.1002/adhm.202202660

Figure Lengend Snippet: Cell type, tissue origin, and reasons for the selection of the cells. Abbreviations: HUVECs: human umbilical vein endothelial cells, hiPSC‐CMs: human induced pluripotent stem cell derived cardiomyocytes

Article Snippet: Human bone MG‐63 osteosarcoma fibroblasts (CRL‐1427, ATCC, USA) were cultured in EMEM supplemented with 10% v/v FCS, 1% v/v GlutaMax, 1 % v/v NEAA, and 0.1% v/v gentamycin sulfate.

Techniques: Selection, Derivative Assay

Journal: Advanced Healthcare Materials

Article Title: Design of Recombinant Spider Silk Proteins for Cell Type Specific Binding

doi: 10.1002/adhm.202202660

Figure Lengend Snippet: Cell adhesion on films made of eADF4(C16) (cytophobic), eADF4(C16)‐RGD (cytophilic), and eADF4(C16)‐KGD (cell selective). Cell adhesion and spreading of rat B50 neuronal cells, mouse Balb 3T3 embryonal fibroblasts, human BJ fibroblasts, mouse C2C12 myoblasts, human HaCaT keratinocytes, human induced pluripotent stem cell derived cardiomyocytes (hiPSC‐CM), human HeLa cervix carcinoma cells, human umbilical vein endothelial cells (HUVEC), human MG63 bone fibroblasts, mouse‐rat NG108 neuronal hybrid cells, and rat RN22 Schwann cells on different spider silk films were recorded after 4 h of incubation using fluorescence microscopy. Therefore, cell nuclei and F‐actin cytoskeleton of fixed cells were stained using DAPI (blue) and Phalloidin (red), respectively. White arrows point towards round cellular aggregates showing small cell body sizes and preferring cell–cell‐contacts, but no/little cell–matrix interactions. White asterisks highlight clearly spread cells exhibiting enhanced cell body sizes. The yellow box marks the selectivity of C2C12 myoblasts on the eADF4(C16)‐KGD variant. Scale bars: 100 µm.

Article Snippet: Human bone MG‐63 osteosarcoma fibroblasts (CRL‐1427, ATCC, USA) were cultured in EMEM supplemented with 10% v/v FCS, 1% v/v GlutaMax, 1 % v/v NEAA, and 0.1% v/v gentamycin sulfate.

Techniques: Derivative Assay, Incubation, Fluorescence, Microscopy, Staining, Variant Assay

Journal: ACS Applied Bio Materials

Article Title: Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

doi: 10.1021/acsabm.3c00126

Figure Lengend Snippet: Shrinkage of the hydrogels with 3D cultured human lung fibroblasts (HLFs). (A) Pictures of Col-I and Col-I/BCf hydrogels completely detached (SB) with and without HLFs. Scale bars: 1 cm. (B, C) Quantification of the reduced area of hydrogels containing HLFs compared to acellular hydrogels while having detached only the sides (B), or sides and bottoms (C) of hydrogels after 7 days of 3D culture. (D) Maximum intensity projection images of 150 μm Z-stacks of live confocal images of stained HLFs after being cultured within the hydrogels for 7 days. Cells were stained with a live/dead kit where live cells appear in green (Calcein-AM) and dead cells in red (EthD-1). * p ≤ 0.05 and ** p ≤ 0.01.

Article Snippet: Human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) and human lung fibroblasts (HLFs) were purchased from ATCC (PCS-500-012) as well as the culture media containing 125 pg/mL recombinant human fibroblast growth factor (rh FGF), 15 ng/mL recombinant human insulin-like growth factor-1, 7% fetal bovine serum (FBS), and 2.4 mM l -alanyl- l -glutamine.

Techniques: Cell Culture, Staining

Journal: Carbohydrate Polymer Technologies and Applications

Article Title: 3D Bioprintable hydrogels via enzymatic crosslinking of hyaluronic acid and phenol-functionalized gelatin to mimic extracellular (ECM) network

doi: 10.1016/j.carpta.2025.100928

Figure Lengend Snippet: Figure 11 viability of hBM-MSCs obtained after cytotoxicity test performed at time points 1, 3 and 7

Article Snippet: For cell experiments, human bone marrow-derived mesenchymal stem cells (hBM-MSCs), mesenchymal stem cell basal medium and mesenchymal stem cell growth kit for bone marrow-derived MSCs (recombinant human Fibroblast growth factor basic, rh FGF-b, recombinant human Insulin-like growth factor 1, rh IGF-1, L-Alanyl-L-Glutamine) were purchased from LGC Standards (Milan, Italy).

Techniques:

Journal: Carbohydrate Polymer Technologies and Applications

Article Title: 3D Bioprintable hydrogels via enzymatic crosslinking of hyaluronic acid and phenol-functionalized gelatin to mimic extracellular (ECM) network

doi: 10.1016/j.carpta.2025.100928

Figure Lengend Snippet: Figure 13 images of hBM-MSCs bioprinted into hydrogels at different time points (A) and viability

Article Snippet: For cell experiments, human bone marrow-derived mesenchymal stem cells (hBM-MSCs), mesenchymal stem cell basal medium and mesenchymal stem cell growth kit for bone marrow-derived MSCs (recombinant human Fibroblast growth factor basic, rh FGF-b, recombinant human Insulin-like growth factor 1, rh IGF-1, L-Alanyl-L-Glutamine) were purchased from LGC Standards (Milan, Italy).

Techniques: